By Sabine Globig

ISBN-10: 1926692810

ISBN-13: 9781926692814

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Tracking of FITC- Labeled ODNs in the Tissue of Rat Uterus Pregnant rats were injected with Hsp105 S-ODNs or A-ODNs according to the procedures described by Zhu et al. [34]. Tracking of FITC-labeled ODNs was performed according to the procedures described by Luu et al. [35]. Uterine penetration of the ODNs and cross-contamination between the two horns were assessed by injecting 10 μg (in 100 μL distilled water) of FITC-A-ODNs or FITC-S-ODNs into one horn, with either unlabeled standard control A-ODNs or S-ODNs alone into the contralateral horn of the uterus.

Human endometrium [27], deciduas [28,29] and trophoblast tissues have been also reported to be capable of expressing Hsps during the first trimester of pregnancy[30,31], however, to the best of our knowledge, no studies about an action of Hsp105 in mammalian uterus during implantation have been reported. In the present study, we have analyzed Hsp105 protein expression in rat uterus of early pregnancy, and examined the effect of injection of antisense Hsp105 oligodeoxynucleotides into the pregnant uterine horn on embryo implantation.

The Guidelines for the Care and Use of Animals in Research enforced by Beijing Municipal Science and Technology Commission were followed. All protocols have been approved by the Animal Care and Use Committee of Institute of Zoology, Chinese Academy of Sciences. The rats were caged in a controlled environment with a 14 hr light:10 hr dark cycle. The adult females were mated with fertile males of the same strain to induce pregnancy (day 1, D1 = day of vaginal plug positive). Pregnancy on D1–5 was confirmed by flushing embryos from the reproductive tracts.

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