By Anthony R. Means, P. Michael Conn (Eds.)

ISBN-10: 0121820394

ISBN-13: 9780121820398

The severely acclaimed laboratory commonplace, Methods in Enzymology, is likely one of the so much hugely revered courses within the box of biochemistry. in view that 1955, each one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate at the present time - actually a necessary ebook for researchers in all fields of lifestyles sciences

Show description

Read Online or Download Cellular Regulators Part A: Calcium- and Calmodulin-Binding Proteins PDF

Best biochemistry books

Get Concise Encyclopedia of Biochemistry and Molecular Biology PDF

Comprises a few 4,500 entries and approximately 1,000 figures, formulation, and tables. Entries comprise citations of modern literature. This 3rd variation bargains many new entries reflecting advances within the realizing of molecular and mobilephone biology, metabolic pathways, and the choice of macromolecular st

Download e-book for iPad: High-Throughput Glycomics and Glycoproteomics: Methods and by Gordan Lauc, Manfred Wuhrer

This quantity discusses specified protocols for the research of glycosylation on the point of unfastened glycans and glycopeptides. The e-book covers issues comparable to the significance of glycans in eukaryotic existence (with a spotlight on mammals and especially humans); contemporary applied sciences that let the characterization of bigger units of samples; and approach repeatability and robustness, in addition to larger all through with appreciate to pattern guidance, dimension and information research.

Additional info for Cellular Regulators Part A: Calcium- and Calmodulin-Binding Proteins

Example text

26) had molecular weights of 30,000 and 33,000 on SDS gels, indicating that they may occur as tetramers in the native state. It is not clear if they are made of identical or nonidentical subunits. But a closer examination of this region shows an initial high concentration of 33K and gradual appearance of 30K proteins. This may suggest that there are two heavy molecular weight forms, each composed of identical subunits. 2) (Fig. 1). Practically all the 33K calcimedin was distributed almost equally between 50 and 150 mM NaC1 eluents while >90% of 30 K calcimedin appeared in 50 mM NaC1 eluent.

Bacterial lysates). Since CaM contains no free sulfhydryl groups, the use of the organomercurial columns selectively removes "nonspecific" protein contaminants which bind melittin-Sepharose as well as thiol-containing proteins exhibiting higher affinity interactions such as S-100 protein. , increasing the ratio of tissue to affinity gel); indeed, a single-step purification from testis supernatant, without organomercurial agarose, was achieved by just saturating the binding capacity of a gel which had been used repeatedly (Fig.

8. All hydrophobic affinity chromatography steps are carried out at 4°. Depending on the tissue source the eluent contains a mix of proteins, the most prominent of which have molecular weights of 32,500 and 67,000. 1% SDS and distilled water, followed by the loading buffer. The individual calelectrins can be purified from the EGTA-eluent by several methods. Gel Filtration. Any column with a separation range of 10 to 100 kDa will be suitable, but we found an AcA 44 column especially effective. 4, the calelectrins behave like monomers on these columns with Stokes radii corresponding to their monomeric molecular weights.

Download PDF sample

Cellular Regulators Part A: Calcium- and Calmodulin-Binding Proteins by Anthony R. Means, P. Michael Conn (Eds.)


by Mark
4.2

Cellular Regulators Part A: Calcium- and Calmodulin-Binding - download pdf or read online
Rated 4.73 of 5 – based on 12 votes