By Sidney Fleischer, Becca Fleischer
The shipping volumes of the Biomembranes sequence have been initiated with Volumes a hundred twenty five and 126 of equipment in Enzymology. those volumes lined delivery in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 hide ATP-Driven Pumps and similar Transport.
The subject of organic membrane delivery is a truly well timed one simply because a robust conceptual foundation for its realizing now exists
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Additional resources for Biomembranes Part Q: ATP-Driven Pumps and Related Transport: Calcium, Proton, and Potassium Pumps
All rights of reproduction in any form reserved. 8 kg weight per rabbit) are killed by cervical dislocation. All subsequent steps are carried out at 0-4 °. The predominantly white muscles [sartorius, gracilis, vastus lateralis, vastus medialis, adductor magnus, glutens (maxima, medius, minimus), biceps femoris, and gastrocnemius] of the hind legs are dissected from red muscle, large blood vessels, nerves, tendon, and fascia. The dissection is performed in a cold room on a glass tray seated on crushed ice.
On the other hand, since Ca 2+ is assayed by contraction, a Mg 2+ concentra22 M. Endo, Physiol. Rev. 57, 71 (1977). 23 M. E n d o , Proc. Jpn. Acad. 51, 479 (1975). 24 B e c a u s e caffeine-induced Ca 2+ release is stronger at higher Ca 2+ concentrations, a very rapid Ca 2÷ release is obtained w h e n the Ca 2+ ion released in turn accelerates a further release o f C a 2÷. 25 M. Endo, Proc. Jpn. Acad. 51, 467 (1975). 0, at 0-5 ° for amphibian fibers and 20-25 ° for mammalian fibers. 2 M for amphibian and mammalian fibers, respectively, by adding an appropriate amount of potassium methanesulfonate.
B. Donaldson and W. G. L. Kerrick, J. Gen. Physiol. 66, 427 (1975). 9 A. Takagi and M. Endo, Exp. Neurol. 55, 95 (1977). to R. Natori, Jikeikai Med. J. 1, 119 (1954).  C a 2+ RELEASE IN SKINNED SKELETAL MUSCLE FIBERS 15 cause Ca 2÷ release by manipulating the T membrane potential. To obtain well-resealed T tubules, skinning in an oil with a minimal quantity of extracellular fluid around the fiber is recommended, since Ca 2÷ seems to be required for the fusion of the disrupted membranes. Chemical Skinning Chemical skinning for Ca 2÷ release experiments should be performed with an agent that destroys the semipermeability of the surface membrane but not that of the SR.
Biomembranes Part Q: ATP-Driven Pumps and Related Transport: Calcium, Proton, and Potassium Pumps by Sidney Fleischer, Becca Fleischer
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