By Yu. M. Torchinsky, T. T. Berezov (auth.), Dr. Timo K. Korpela, Prof. Philipp Christen (eds.)

ISBN-10: 3034893086

ISBN-13: 9783034893084

ISBN-10: 3034899890

ISBN-13: 9783034899895

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Extra info for Biochemistry of Vitamin B6 : Proceedings of the 7th International Congress on Chemical and Biological Aspects of Vitamin B6 Catalysis, held in Turku, Finland, June 22–26, 1987

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J. (1985) J. Biol. Chem. 260, 9346-9356. V. (1973) FEBS Lett. 29, 31-34. , and wU; R. (1981) J. Biol. Chem. 256, 6480-6486. , and Gehring, H. (1986) J. Biol. Chem. 261, 11146-11149. , and Gehring, H. (1982) J. Biol. Chem. 257, 3339-3345. , Jaussi,~, and Christen, P. (1985) In: Transaminases (P. E. Metzler, Eds) Wiley, New York, pp. 502-510. M. (1986) Ann. Rev. Biochem. 55, 249-285. BCR/LS 2 Biochemistry of Vitamin B6 (c) 1987 Birkhauser Verlag Basel 31 STRUCTURE-FUNCTION RELATION OF THE PRESEQUENCE OF A PRECURSOR TO PIG MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE T.

About one third of pre-mAspAT appears to be digested by lysosomes. Secondary cultures of chicken embryo fibroblasts, if grown under pyridoxal depletion, contain N30 per cent of total mAs pAT in its apo form. Double isotope-labeling experiments showed that the apoenzyme corresponds to newly synthesized protein. Apparently, the translocation of the precursor into mitochondria and its processing proceed independently of the coenzyme. INTRODUCTION Mitochondrial proteins are of twofold origin. e. about 90 per cent of their protein mass, are encoded by genes in the nucleus of the cell, synthesized in the cytosol, and imported into the mitochondria (for a recent review, see Tzagoloff and Myers, 1986).

In press. , and Morino, Y. (1985) Proc. Natl. Acad. Sci. USA 82, 6065-6069. E. (1975T J. Biol. Chem. 250, 5604-5608. H. (1986) Nucleic Acid Res. 14, 1379-1391. J. (1985) J. Biol. Chem. 260, 9346-9356. V. (1973) FEBS Lett. 29, 31-34. , and wU; R. (1981) J. Biol. Chem. 256, 6480-6486. , and Gehring, H. (1986) J. Biol. Chem. 261, 11146-11149. , and Gehring, H. (1982) J. Biol. Chem. 257, 3339-3345. , Jaussi,~, and Christen, P. (1985) In: Transaminases (P. E. Metzler, Eds) Wiley, New York, pp. 502-510.

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Biochemistry of Vitamin B6 : Proceedings of the 7th International Congress on Chemical and Biological Aspects of Vitamin B6 Catalysis, held in Turku, Finland, June 22–26, 1987 by Yu. M. Torchinsky, T. T. Berezov (auth.), Dr. Timo K. Korpela, Prof. Philipp Christen (eds.)


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