By A. Fallon, R.F.G. Booth and L.D. Bell (Eds.)

ISBN-10: 0444808620

ISBN-13: 9780444808622

This booklet is meant to familiarize biochemists with HPLC. Theoretical elements of every mode of chromatography are mentioned in chapters 1-9, offering an figuring out of some of the modes of chromatography that are now attainable utilizing commercially to be had columns, from reversed section to affinity. useful facets and instrumentation are lined in bankruptcy 10. the majority of the e-book, which follows, offers examples and functions of every mode of chromatography in present biochemical perform.

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Fraction collectors A variety of fraction collectors are available from a number of manufacturers and include those which are able to collect low microlitre volumes in addition to those used in preparative HPLC which can collect much larger volumes. Fraction collectors which are to be used with HPLC systems require very rapid tube changing facilities and should also be resistant to the solvents used with HPLC systems; thus, fraction collectors which are designed for use with low pressure chromatography are usually inadequate.

E) Check that no air bubbles are present in the flow cell. (f) Ensure that all constituents of the mobile phase are of the highest quality. ( g ) Passivate the HPLC system regularly. Ch. 8. Fraction collectors A variety of fraction collectors are available from a number of manufacturers and include those which are able to collect low microlitre volumes in addition to those used in preparative HPLC which can collect much larger volumes. Fraction collectors which are to be used with HPLC systems require very rapid tube changing facilities and should also be resistant to the solvents used with HPLC systems; thus, fraction collectors which are designed for use with low pressure chromatography are usually inadequate.

I . Introduction Ion-exchange chromatography was the first mode of chromatography to be developed to the point that we now term high performance liquid chromatography. The advances began in the early 1950s with a requirement to improve amino acid analysis techniques. The pressures used were initially quite low (about 100 psi, the working limit of glass columns). The same requirement was later demanded for the analysis of many other biochemical compounds, such as nucleotides in urine and blood. The chromatographic supports were based on spherical beads of polystyrene-divinylbenzene copolymer resins which were gradually made with smaller and smaller diameters with the aim of improving performance.

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Applications of HPLC in Biochemistry by A. Fallon, R.F.G. Booth and L.D. Bell (Eds.)


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